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KMID : 0613820030130040416
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Journal of Life Science
2003 Volume.13 No. 4 p.416 ~ p.420
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A Case of Parentage Testing in Dog by Microsatellite DNA Typing
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Cho Gil-Jae
Cho Byung-Wook
Lee Kil-Wang
Kim Seon-Ku
Kim Yong-Gyun
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Abstract
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We performed this study to determine the parentage verification of putative dogs. A total of 7 samples (4 Labrador Retriever dog and 3 Poongsan dog) were genotyped by using 12 international markers (PEZ1, PEZ5, PEZ8, PEZ10, PEZ11, PEZ12, PEZ17, PEZ20, PEZ21, FHC2010, FHC2054, FHC2079). This methods consisted of multiplexing PCR procedures, and it showed reasonable amplification of all PCR products. Genotyping was performed with an ABI 310 genetic analyzer. Labrador Retriever Pup Ⅰand Pup Ⅱ were included according to principles of Mendelian genetics in all loci, while Poongsan Pup Ⅲ was excluded with markers PEZ1 (106/118), PEZ10 (276/300), and FHC2010 (228/ 232). These results suggest that the present DNA typing is so useful for parentage verification of these two breeds.
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KEYWORD
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dog, microsatellite, parentage verification
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